Antioxidant effect of persimmon leaf extract to HEK293-APPswe transgenic cells and effect to Nrf2/HO-1 pathway
10.3969/j.issn.1000-484X.2017.06.010
- VernacularTitle:柿叶提取物对HEK293-APPswe转基因细胞模型的抗氧化作用及对Nrf2/HO-1途径的影响
- Author:
Xiaofan WU
;
Bin MA
;
Xunyao HOU
;
Yan HONG
;
Chao SHEN
;
Xueping LIU
- Keywords:
Persimmon leaf extract (PLE);
Oxidative stress;
Neuroprotection;
Nrf2/HO-1
- From:
Chinese Journal of Immunology
2017;33(6):854-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of persimmon leaf extract (PLE) on HEK293-APPswe transgenic cells (20E2).Methods:To determine whether the 20E2 cells model was successfully established,the level of Aβ1-40 in SH-SY5Y was detected and 20E2 cells(HEK293 cells stably expressing Swedish mutant APP)cultured in vivo by ELISA kit,and the expression of APP protein level was detected by Western blot.Cell viability was assayed by CCK-8 method and then selected the best concentration.Set groups:SH-SY5Y as normal control group (NC group),20E2 as model group (20E2 group),treating with PLE as treating group (20E2+PLE group).Reactive oxygen species (ROS) levels of each group were detected with DCFH-DA fluorescent probe.The extracellular level of Aβ1-42 were detected by ELISA kit.Cytoplasmic Nrf2,Nuclear Nri2,Whole-cell HO-1 were detected by Western blot.Results:Compared with model group,the expressions of ROS,Aβ1-42 were down-regulated and the Nuclear Nrf2 and Whole-cell HO-1 were up-regulated in 20E2+PLE group.Conclusion:PLE can reduce the level of oxidative stress of model group effectively,it possibly reduce the aggregation of Aβ1-42 and prevent oxidizing via activating Nrf2/HO-1 pathway.
