Quercetin ameliorates postoperative pain by suppressing matrix metalloproteinase in microglia
10.11665/j.issn.1000-5048.20170315
- VernacularTitle:槲皮素抑制脊髓小胶质细胞基质金属蛋白酶的表达改善术后疼痛的机制研究
- Author:
Yixin FAN
;
Haijiao LIU
;
Yixuan LI
;
Yan ZHANG
;
Wentao LIU
;
Guangqin ZHANG
- Keywords:
quercetin;
MMP-9;
MMP-2;
microglia;
postoperative pain
- From:
Journal of China Pharmaceutical University
2017;48(3):343-347
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the effects and mechanisms of quercetin on plantar incision-induced matrix metalloproteinase (MMP)-9 and MMP-2 activity,microglia activation and the analgesic effect of quercetin on plantar incision surgery-treated mice.Postoperative pain model was mediated by plantar incision surgery and Von Frey Hairs was used to test the mechanical pain threshold.The activity of MMP-9 and MMP-2 in spinal cord was evaluated by gelatin zymography.The marker of microglia ionized calcium binding adapter molecule 1 (IBA-1),phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) was detected by Western blot.Results showed that quercetin (20,40,80 mg/kg,ip) significantly inhibited plantar incisioninduced mechanical allodynia and suppressed the activity of MMP-9 and MMP-2 in the spinal cord.Moreover,quercetin also markedly inhibited plantar incision-induced up-regulation of IBA-1 and p-p38 in spinal cord.In conclusion,quercetin may alleviate postoperative pain by suppressing MMP-9 and MMP-2 activity in microglia.
