Effect of Surface Charge on Biobehavior ofPEGylated Gold Nanoparticles
10.11895/j.issn.0253.3820.170085
- VernacularTitle:表面电荷对聚乙二醇修饰金纳米粒子生物行为的影响
- Author:
Yu FU
;
Xiaodong LI
;
Zhuheng LI
;
Hongda CHEN
;
Zhenxin WANG
;
Huimao ZHANG
;
Haifeng WANG
- Keywords:
PEGylated;
Gold nanoparticles;
Living cells;
Biodistribution;
Biocompatibility.
- From:
Chinese Journal of Analytical Chemistry
2017;45(7):1038-1044
- CountryChina
- Language:Chinese
-
Abstract:
Three kinds of PEGylated gold nanoparticles (PEG-Au NPs) with different surface charges are prepared by assembly of thiolated polyethylene glycol (HS-PEG) with different terminal groups including methoxy, amino or carboxyl on gold nanoparticle surface through sulfur-gold covalent bond, respectively.The experimental results of cell co-culturing and tail intravenous injection in mouse indicate that the biological behaviors of PEG-Au NPs are affected significantly by their surface charges.The cellular internalization amounts of PEG-Au NPs are following the order, positive charge ＞ neutral charge ＞ negative charge.The PEG-Au NPs are gradually transferred to liver and spleen from main organs through the circulation of blood after tail intravenous injection in mouse.The negatively charged PEG-Au NPs have the slowest hepatic clearance rate while the positively charged PEG-Au NPs can cause the strongest response of immune system in mice.
