Monitoring of Subthalamic Nucleus Stimulation-induced DopamineFlux and Spike Firing Change of Striatum Neuronswith a Micro-Nano Electrode Array
10.11895/j.issn.0253.3820.170116
- VernacularTitle:微纳电极阵列检测丘脑底核电刺激引起的纹状体神经元活动变化
- Author:
Lili YANG
;
Yilin SONG
;
Shengwei XU
;
Yu ZHANG
;
Guihua XIAO
;
Song ZHANG
;
Fei GAO
;
Ziyue LI
;
Xinxia CAI
- Keywords:
Micro-electromechanical systems;
Micro-nano electrode array;
Subthalamic nucleus stimulation;
Dopamine;
Spike
- From:
Chinese Journal of Analytical Chemistry
2017;45(7):1088-1095
- CountryChina
- Language:Chinese
-
Abstract:
Subthalamic nucleus (STN) deep brain stimulation (DBS) has become an important surgical treatment of Parkinson disease, but its exact mechanism is still unclear.In this study, a 16-channel implantable microelectrode array (MEA) was prepared by micro-electromechanical system (MEMS) technique and later modified with platinum black/reduced Graphene Oxide/Nafion (Pt/RGO/Nafion) nanocomposites.Extracellular dopamine (DA) content and spike of dorsal striatum neurons were synchronously recorded before and after STN stimulation.The results showed that the dopamine content began to increase within 20 s after electrical stimulation and dropped to normal level after about 50 s, with the highest rising concentration of 1.72 μmol/L.At the same time, there was an increased spike activity of interneurons in the dopamine ascending phase, and the spike firing rate of medium spiny projection neurons (MSNs) was high when the concentration of DA was higher than the normal level.The MEA sensor can simultaneously record dopamine flux and physiological signals in situ, thus providing an ideal tool for neural information detection.
