Determination of Nicotinate-curcumin Ester and Its Nanoparticles by HPLC
- VernacularTitle:HPLC法测定烟酸姜黄素酯及其纳米粒的含量
- Author:
Jingyi GUO
;
Man HU
;
Peng WANG
;
Duanfang LIAO
;
Limei LIN
;
Qun HE
- Keywords:
Nicotinate-curcumin ester;
Nanoparticles;
HPLC;
Content determination
- From:
China Pharmacist
2017;20(7):1181-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To provide basis for the preparation process study by establishing an HPLC determination method for nicotinate-curcumin ester.Methods: A C18 column(250 mm×4.6 mm,5 μm) was used at 30℃ with the mobile phase of acetonitrile-0.5% acetic acid glacial(65∶35) at a flow rate of 1.0 ml·min-1.The UV detection wavelength was 280 nm.Results: The good linear relationship of nicotinate-curcumin ester was shown within the range of 5.200-104.000 μg · ml-1(r=0.9999 4), The average recovery of nicotinate-curcumin ester and its nanoparticles was 100.1% and 100.9%with RSD of 1.260% and 0.995 0%(n=6), respectively.Conclusion: The method is convenient, accurate and reproducible, and suitable for the determination of nicotinate-curcumin ester nanoparticles and the studies on the preparation technology.
