Simultaneous Determination of Five Components in Compound Paracetamol Vitamin C Yinqiao Tablets by HPLC-DAD
- VernacularTitle:HPLC-DAD法同时测定复方银翘氨酚维C片中5种组分的含量
- Author:
Shu JIN
- Keywords:
HPLC-DAD;
Compound paracetamol vitamin C Yinqiao tablets;
Phillyrin;
Chlorogenic acid;
Acetaminophen;
Chlorphenamine;
Vitamin C
- From:
China Pharmacist
2017;20(7):1305-1307
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To simultaneously detect five components (phillyrin, chlorogenic acid, chlorpheniramine maleate, vitamin C and acetaminophen) in compound paracetamol vitamin C Yinqiao tablets.Methods: Using a Waters Xselect C18 column(250 mm×4.6 mm, 5 μm), the mobile phase consisted of acetonitril-0.1 mol·L-1 NaH2PO4(pH 3.0) with gradient elution at a flow rate of 1.0 ml·min-1.The detection wavelength was 230 nm and the column temperature was 35℃.Results: The linear range of vitamin C, acetaminophen, chlorogenic acid, chlorphenamine maleate and phillyrin was 0.500-200.200, 0.499-199.600, 0.501-200.100, 0.502-200.900 and 0.488-195.400 μg·ml-1(r＞0.999), respectively.The average recovery was 99.3%(RSD=1.5%), 97.8%(RSD=1.7%), 98.5%(RSD=1.1%), 97.1%(RSD=1.7%)and 99.8%(RSD=1.2%)(n=9), respectively.Conclusion: The simple and sensitive method can be applied in the quality control of compound paracetamol vitamin C Yinqiao tablets.
