Simultaneous determination of five furostanol saponins in rhizome and fibrous root of Anemarrhena asphodeloides Bge. by HPLC-CAD
10.13220/j.cnki.jipr.2017.06.033
- VernacularTitle:HPLC-CAD同时测定知母根茎与须根中5种呋甾皂苷的含量
- Author:
Fengxia MA
;
Xinguang SUN
;
Jie ZHANG
;
Zhen LONG
;
Yang ZHAO
;
Chunni ZHANG
;
Fangxu WANG
;
Shuchen LIU
;
Baiping MA
- Keywords:
HPLC with the charged aerosol detector;
Anemarrhena asphodeloides Bge.;
furostanol saponin;
simultaneous de-termination
- From:
Journal of International Pharmaceutical Research
2017;44(6):642-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the determination of five furostanol saponins(timosaponin N,timosaponin L, timosaponin BⅡ,25R-timosaponin BⅡ,and 25S-officinalisnin-Ⅰ)in rhizome and fibrous root of Anemarrhena asphodeloides Bge. by HPLC with the charged aerosol detector(CAD). Methods The analysis was performed on TechMate C18-ST-II(250 mm×4.6 mm,5μm)with acetonitrile:water(22:78,V/V),the flow rate of 1.0 ml/min and column temperature at 30℃. The Corona parameters were as follows:sampling rate 10 Hz,filter 5 s,and the nebulizer temperature 55℃. Results The approach showed good linearity for five saponins. The correlation coefficients(r2)for calibration curves varied from 0.9992 to 0.9998. The limits of detection(LOD)were 0.28,0.92,0.92,0.92 and 0.92 ng for five steroidal saponins,respectively. The limits of quantitation(LOQ)were found to be 0.92, 2.77,2.77,2.77 and 2.76 ng,respectively. RSD calculated from peak area of precision,repeatability and stability in 48 h were all less than 3.0%. The average recoveries of timosaponin N,timosaponin L,timosaponin BⅡ,25R-timosaponinBⅡ,and 25S-officinalis-nin-Ⅰwere 98.17%,101.37%,98.53%,97.63%,and 98.17%,respectively. Conclusion The developed method is accurate,reli-able,which could be applied to the quality control of multiple components in A. asphodeloides Bge.
