Inhibitory effect of human embryonic stem cells on HepG2 cells in vitro
10.3969/j.issn.1000-484X.2017.06.012
- VernacularTitle:人胚胎干细胞对肝癌HepG2细胞体外抑制作用的研究
- Author:
Liangdong ZHENG
;
Xuemei HE
;
Ting ZHANG
;
Jie LIU
;
Bennian HUO
;
Mengnan LIU
;
Xue WANG
;
Tao FENG
- Keywords:
Embryonic stem cells;
Hepatoma;
Co-culture;
Inhibition;
Apoptosis
- From:
Chinese Journal of Immunology
2017;33(6):864-868
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of human embryonic stem cells on the HepG2 cells in vitro.Methods:The co-culture system of Human embryonic stem cells (H9) and liver cancer HepG2 cells was established.The effect of H9 on the biological behavior of HepG2 cells was observed by microscope,the flow cytometry was used to detcct the apoptosis of tumor cells and the cell cycle alteration.Transwell assay was used to detect the migration and invasion of tumor cells.Gene microarray technique was used to examine the change of gene expression profile of HepG2 cells.Results:In the process of co-culture,the growth of hepatoma cells was inhibited.With the extension of the culture time,cells decreased gradually,and occurred signs of aging or apoptosis.Flow cytometry test results showed that the apoptosis rate of hepatoma ceils was significantly increased,and the cell cycle was blocked in the G0/G1 phase.Transwell test results showed that the invasion and migration of HepG2 cells were decreased.The gene chip results showed that the whole genome expression profile of HepG2 cells had a significant change.Conclusion:The human embryonic stem cells had an inhibitory effect on HepG2 cells in vitro.
