miR-410 expression and bioinformatics analysis of its predicted target genes in systemic lupus erythematosus
10.3969/j.issn.1000-484X.2017.06.016
- VernacularTitle:系统性红斑狼疮患者中miR-410表达及其靶基因生物信息学分析
- Author:
Ji ZHANG
;
Daming OU
;
Lifang HUANG
;
Yanglin OU
;
Liheng ZOU
- Keywords:
miR-410;
SLE;
Target gene;
GO enrichment;
KEGG pathway
- From:
Chinese Journal of Immunology
2017;33(6):884-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect expression level of miR-410 in patients with systemic lupus erythematosus (SLE),and to expose the role of miR-410 and its target genes by bioinformatics methods.Methods:Expression level of miR-410 were detected by quantitative RT-PCR in peripheral blood mononuclear cells of SLE patients,and miR-410 sequence,its target genes and Genecards database were analyzed,and analysis of GO enrichment and KEGG Pathway was further performed.Results:miR-410 expression was significantly reduced in SLE patients,and its nucleotide sequence was highly conserved among species.These genes that were predicted to be regulated by miR-410 and associated with LE pathogenesis,included FASLG,CSF2,IFNAR2,MAPK1,PLCG2,IL4 and other genes.Analysis of GO enrichment revealed that miR-410's target genes were involved in cell growth,proliferation,programmed cell death,cell differentiation,immune system development and other biological activities.Analysis of KEGG Pathway showed that the target genes of miR-410 were significantly enriched in a series of signaling pathways including pathways in cancer,cytokine-cytokine receptor interaction,glioma,melanoma,TGF-β and JAK-STAT signaling pathway.Conclusion:miR-410 maybe directly regulate its target molecules,mediate various signal pathway networks,thus participate in the occurrence and development of SLE.
