Liver-targeted expression of chemokine CXCL10 inhibits ConA-induced liver injury
10.3969/j.issn.1000-484X.2017.06.005
- VernacularTitle:肝脏靶向表达趋化因子CXCL10抑制ConA诱导的肝脏损伤
- Author:
Jing LI
;
Xuefu WANG
- Keywords:
Concanavalin A;
Liver injury;
CXCL10;
Inflammatory cytokine
- From:
Chinese Journal of Immunology
2017;33(6):828-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of CXCL10 on immune-mediated liver injury.Methods:The CXCL10 expression vector was injected into mice by hydrodynamic injection.The levels of CXCL10 in the liver were measured 72 hours after injection.Concanavalin A (ConA) was injected into mice to induce immune-mediated liver injury.The levels of alanine a minotransferase (ALT),tissue damage,IFN-γ,TNF-α and percentages and numbers of regulatory T cells were tested and compared between CXCL10 expression group and control group.Results:The levels of CXCL10 in the liver were significantly increased at 72 hours after CXCL10 expression vector was injected.The pretreatment of CXCL10 expression vector markedly reduced ConA-induced ALT levels,IFN-γlevels and TNF-α levels.Additionally,the pretreatment of CXCL10 expression vector increased the percentages and numbers of regulatory T ceils in the liver.Conclusion:The pretreatment of CXCL10 expression vector decreases the levels of inflammatory cytokines and attenuates ConA-induced liver injury,which might be beneficial for the treatment for immune-mediated liver injury.
