Helicobacter pylori combined with MNU gavage for preparing balb/c mouse gastric cancer model
10.3969/j.issn.1671-8348.2017.20.023
- VernacularTitle:幽门螺杆菌联合MNU灌胃法制备balb/c小鼠胃癌模型研究
- Author:
Yanzhen SUN
;
Tao ZHANG
;
Liangrong CHEN
;
Chao OU
;
Xiaoyan HUANG
;
Jie YANG
;
Yuanneng CHENG
- Keywords:
N-methyl-N-nitrosourea;
Helicobacter pylori;
balb/c mice;
stomach neoplasms
- From:
Chongqing Medicine
2017;46(20):2806-2808,2811
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Helicobacter pylori combined with N-methyl-N-nitrosourea (MNU) gavage for preparing balb/c mouse gastric cancer model.Methods Eighty balb/c mice were randomly divided into four groups after 1-week adaptive feed,normal group,model group Ⅰ,Ⅱ and Ⅲ,20 cases in each group.The model group Ⅰ,Ⅱ and Ⅲ were given Helicobacter pylori bacteria liquid (CFU=109/mL) gavage,once every other day for 5 times;then,the freshly configured MNU solution 0.15,0.3,0.6 mL gavages were in turn given,MNU and pure water allocation ratio was 5mg:3mL.Once gavage per week for continuous 10 weeks.Results The model group II had 66.67% adenocarcinoma,the model group I were gastritis with mild atypical hyperplasia,and all mice in the model group III died.Conclusion This method can prepare the gastric cancer model.
