Study on content determination of lobetyolin and gallic acid in Eighteen Flavors Dangshen Pill from different factories
10.3969/j.issn.1671-8348.2017.20.024
- VernacularTitle:高效液相法对不同厂家十八味党参丸中党参炔苷及没食子酸水平的检测研究
- Author:
Zixuan LI
;
Yong LI
;
Chao LUO
;
Caijuan BAI
;
Sang ZHA
;
Lijuan NIE
- Keywords:
eighteen flavors dangshen pill;
obetyolin;
gallic acid
- From:
Chongqing Medicine
2017;46(20):2809-2811
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for determining the contents of lobetyolin and gallic acid in Eighteen Flavors Dangshen Pill(EFDSP) produced by different factories.Methods The HPLC analysis was performed on a VP-DOS C18 column (4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile and 0.4% glacial acetic acid(21∶79) in the determination of lobetyolin content.The detection wavelength was 267 nm and the flow velocity was 1 mL/min.the column temperature was25 ℃ and the sample size was10 μL.The mobile phase was methanol and 0.4% glacial acetic acid(1∶99) in the determination of gallic acid content.The detection wavelength was 280 nm.The column temperature was 25 ℃ and the sample size was 10μL.Results The contents of lobetyolin and gallic acid in EFDSP were 1.0835mg·g-1and 15.334 0 mg/g for Qinghai Gela Dandong Tibetan Pharmaceutical Factory;0.628 9 mg/g and 15.159 5 mg/g for Changdu Tibet Medicine Factory;0.306 5 mg/g and 8.762 7 mg/g for Tibetan Hospital of Tibet.Conclusion This method has the advantages of good reproducibility,good accuracy,simple and fast operation.The contents of lobetyolin and gallic acid in EFDSP produced by different manufacturers are significantly different.The gallic acid content has greater difference.It provides the reference for quality control of EFDSP
