Preparation method of hyperoside from Flos Abelmoschus manihot
10.3760/cma.j.issn.1673-4246.2017.08.015
- VernacularTitle:黄蜀葵花中金丝桃苷的提取分离及纯度检测
- Author:
Shunli XIAO
;
Min WANG
;
Qi LI
;
Qingyun CAO
;
Rongkai GAO
;
Honggui ZHANG
- Keywords:
Hyperin;
Extraction;
Isolation & Purification;
Chromatography,high pressure liquid
- From:
International Journal of Traditional Chinese Medicine
2017;39(8):733-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preparation method and analytical technique of hyperoside from Flos Abelmoschus manihot.Methods Hyperoside was isolated and purified by solvent extract and chromatography, whose structure was determined by 1H-NMR and 13C-NMR. The purity was analyzed by TLC and HPLC.Results The TLC showed that the hyperoside had no impurity spot. The HPLC indicated that the purity reached more than 98.5%.Conclusions The mothod of isolation and purification for hyperoside reported in this paper was simple and economical.
