Effects comparison of chemical hypoxia induced by cobalt chloride and hypoxia environment on rat pulmonary arterial fibroblasts
10.3969/j.issn.1671-8348.2017.21.003
- VernacularTitle:氯化钴化学模拟低氧与低氧环境对大鼠肺动脉成纤维细胞的作用比较
- Author:
Xiaoyu CHAI
;
Huiying XU
;
Zhonghui LIU
;
Liang YI
;
Xinmin LIU
- Keywords:
cobalt chloride;
hypoxia;
pulmonary artery;
fibroblasts;
phenotype transformation
- From:
Chongqing Medicine
2017;46(21):2889-2891,2894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of cobalt chloride (CoCl2) induced chemical hypoxia and hypoxia environment on the proliferation,migration and phenotype transformation of rat primary pulmonary artery fibroblasts (PAFs).Methods Primary PAFs were isolated and cultured.Cells were stimulated by CoCl2,or hypoxia cell culture (1% O2) was used to stimulate and induce PAFs.Then the effects of CoCI2 and hypoxia environment on PAFs were compared by CCK-8 assay,scratch assay,transwell assay,phenotype marker protein expression and PI3K/Akt signaling pathway protein expression.Results Compared with the control group,100 μmol/mL CoCl2 stimulation had no significant effect on the cell proliferation activity,cell migration ability and phenotype transformation ability of PAFs (P>0.05);while 1% O2 could significantly improve the cell proliferation and migration activities of PAFs as well as the upregulation of α-SMA expression (P<0.05).Conclusion There exist differences of effects between CoCl2 induced chemical hypoxia and hypoxia environment on promoting cell proliferation, cell migration and phenotype transformation in PAFs.
