Influence of extracorporeal photochemotherapy-treated regulatory dendritic cells on T cell proliferation
10.11855/j.issn.0577-7402.2017.08.07
- VernacularTitle:体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响
- Author:
Guosheng DU
;
Shaozhen YANG
;
Yuxiang WEI
;
Longlong SUO
;
Likui FENG
;
Jiyong SONG
;
Zhidong ZHU
- Keywords:
extracorporeal photochemotherapy;
dendritic cells;
transplantation immunity
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(8):698-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of regulatory dendritic cells treated by extracorporeal photochemotherapy on T cell proliferation. Methods Human peripheral blood mononuclear cells (PBMCs) were obtained and the immature dendritic cells (imDCs) were induced by recombinant human granulocyte and macrophage colony stimulating factors. SPDCs were obtained by PUVA treatment, and ECDCs were co-cultured with imDCs and PUVA-SP to obtain immunoprecipitated dendritic cells. In vitro, imDCs were co-cultured with SPDCs to obtain SPDCs; imDCs were added to 10ng/ml of LPS, and cultured for 1 day to obtain DCs. The expressions of CD11c, CD83 and CD86 on the surface of the cells were detected. The effect of imDCs on the proliferation of recipient T cells was detected by mixed lymphocyte culture method. Results The early apoptosis rate of PUVA-treated cells was 91.33%. The positive expression rates of CD83 and CD86 in ecpDCs were 22.83%±5.26% and 22.06%±4.37%, respectively, which were similar to those of imDCs (15.06%±0.59%, 15.19%±1.83% (P<0.01), but significantly lower than those in DCs (99.79%±0.36%, 99.85%±0.19%, respectively), the difference was statistically significant (P<0.01). The recipient imDC cells phagocyting the appoptotic splenic lymphocytes from the donor significantly inhibited the proliferation of recipient T cells. Conclusion Apoptosis of splenic lymphocytes induced by extracorporeal photochemotherapy can inhibit the maturation of dendritic cells and inhibit the proliferation of T lymphocytes.
