Optimization of extraction technology for Fufang-Fuqing-Xiji by orthogonal test combined with Plackett-Burman experimental design

VernacularTitle:Plackett-Burman试验设计与正交试验相结合优化复方肤清洗剂提取工艺
Author: Yu ZHU ; Xiaoyan ZHANG ; Juan WEI ; Can ZHOU ; Xia LUO
Keywords: Extraction; Orthogonal test; Plackett-Burman experimental design; Multi-index comprehensive score
From: International Journal of Traditional Chinese Medicine 2017;39(8):724-728
CountryChina
Language:Chinese
Abstract: Objective To optimize extraction technology of Fufang-Fuqing-Xiji.Methods Orthogonal test combined with Plackett-Burman experimental design was adopted to study the influence factors of water extraction in Fufang-Fuqing-Xiji. The contents of chlorogenic acid, paeoniflorin and caffeic acid in Fufang-Fuqing-Xiji were determined by HPLC gradient elution method at the same time. A multi-index comprehensive score method was applied to analyze the data and to screen the optimum extraction technology. Results The best extraction conditions of Fufang-Fuqing-Xiji were as following: threaded 80 mesh sieve, soaked 2 h, decocted 3 times with 12 times the amount of water for 1h each time.Conclusions This optimized extraction technology was rational and stable, which could be used for the extraction ofFufang-Fuqing-Xiji.