Determination of baicalin and naringin inQinbei mixture by HPLC

VernacularTitle:高效液相色谱法测定芩倍合剂中黄芩苷、柚皮苷含量
Author: Yu SUN ; Linhua ZHANG ; Di JIANG ; Zhaozhou LIN ; Daqian WANG
Keywords: Baicalin; Naringin; Chromatography,high pressure liquid; Quality control; Qinbei mixture
From: International Journal of Traditional Chinese Medicine 2017;39(8):729-732
CountryChina
Language:Chinese
Abstract: Objective To establish a high performance liquid chromatography (HPLC) method for the determination of baicalin and naringin inQinbei mixture.Methods The HPLC system consisted of the Fortis-C18(4.6 mm × 250 mm, 5μm) column, and the mobile phase consisted of MeOH:0.4% H3PO4 (42:58), and the flow rate was 1.0 ml/min, and the UV detector was set at 280 nm, and the column temperature was 30℃.Results The linear response range of baicalin was 0.062-0.930μg. The linear response range of naringin was 0.033-0.492μg. The average recovery of baicalin was 98.11% (RSD=1.62%). The average recovery of naringin was 96.78% (RSD=1.74%).Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of baicalin and naringin inQinbei mixture.