Rapid detection of influenza A/H1N1 virus by polymerase spiral reaction

VernacularTitle:聚合酶螺旋反应快速检测甲型H1N1流感病毒
Author: Wen MA ; Derong DONG ; Dayang ZOU ; Ningwei LIU ; Xiaoming HE ; Da AO ; Zhan YANG ; Simo HUANG ; Yaqing XU ; Wei LIU ; Liuyu HUANG
Keywords: polymerase spiral reaction; nucleic acid isothermal amplification; influenza A virus; H1N1 subtype; rapid detection
From: Military Medical Sciences 2017;41(6):449-452
CountryChina
Language:Chinese
Abstract: Objective To establish a polymerase spiral reaction (PSR) method for rapid detection of influenza A/H1N1 virus.Methods Six sets of primers were designed for influenza A/H1N1 virus HA gene, and the results were determined with real time kinetic turbidimetric assay and colorimetry method.Results and Conclusion The best primers were selected from six sets of primers, and the best temperature was determined as 65 degrees Celsius.Further experiments showed that the best primer had good specificity for detection of influenza A/H1N1 virus,without cross reactions with 14 other respiratory tract pathogenic nucleic acids.The sensitivity was up to 100 copies,and consistent with that of PCR.So a PSR method is established for rapid detection of the influenza A/H1N1 virus, which is simple, quick, highly specific and sensitive,and especially applicable to field and grass-roots units.