A new method for screening latent tuberculosis infection in Beijing army recruits
10.7644/j.issn.1674-9960.2017.06.012
- VernacularTitle:驻京某部队新兵结核潜伏感染人群筛查新方法研究
- Author:
Yunlin ZHANG
;
Xuejuan BAI
;
Yan LIANG
;
Jingyu GUO
;
Guoying WANG
;
Liu HE
;
Shumei YANG
;
Xueqiong WU
- Keywords:
latent tuberculosis infection;
fusion protein CFP10-ESAT6;
protein Rv2628;
IGRA;
diagnosis of LTBI
- From:
Military Medical Sciences
2017;41(6):462-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Mycobacterium tuberculosis infections in 2014 among Beijing army recruits, and evaluate a new method for screening latent tuberculosis infections.Methods A total of 194 army recruits were subjected to chest X-ray examination purified protein derivative(PPD) skin test, antibody detection, and interferon gamma release assay(IGRA) by ELISA combined with recombinant protein CFP10-ESAT6 and latent infection protein Rv2628.Results The positive rates of PPD skin test and antibody test were 49.7% and 15.5%, respectively.The latent infection rate of IGRA test was 22.2% in 194 cases after CFP10-ESAT6 stimulation.After stimulation of latent tuberculosis infection(LTBI) with Rv2628, IFN-γ level was significantly higher than that in healthy control group (P<0.05).The weak positive group of TST (5 mm≤diameter<15 mm) had a significantly higher level of IFN-γ than the strong positive group(diameter≥15 mm)(P<0.05),but after stimulation with CFP10-ESAT6,IFN-γ levels were not significantly different between the two groups(P>0.05).There was no significant difference between antibody negative and positive groups after stimulation by CFP10-ESAT6 and Rv2628 (P>0.05).The area under the ROC curve of Rv2628 diagnosis of tuberculosis infection was 0.84.When Youden index was 0.621,the specificity was 94.7% and sensitivity was 67.4%.ConclusionCombined detection of antigens Rv2628 and CFP10-ESAT6 specific IFN-γ values can be potentially used for differential diagnosis of active or latent tuberculosis infections.
