The expression and function of miR-145 in papillary thyroid carcinoma
10.3760/cma.j.issn.1674-6090.2017.03.012
- VernacularTitle:miR-145在甲状腺乳头状癌中的表达及功能研究
- Author:
Xinwei HE
;
Daozhe LIN
;
Dixin XUE
;
Meizhen LIANG
- Keywords:
miR-145;
Papillary thyroid carcinoma;
Proliferation;
Apoptosis
- From:
Chinese Journal of Endocrine Surgery
2017;11(3):224-227,248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and function of miR-145 in papillary thyroid carcinoma (PTC).Methods PTC tissues and adjacent tissues were collected from 43 cases.Expression of miR-145 in PTC tissues and adjacent tissues was detected with RT-PCR.miR-145 analogue was used to transfect TPC-1cell to upregulate miR-145 expression.Brdu-ELISA method was used to detect the proliferation of TPC-1 cell.Flow cytometry instrument was used to detect the apoptosis and cell cycle of TPC-1 cell.Results RT-PCR test showed that relative expression of miR-145 in thyroid carcinoma tissue was 0.369±0.082,significantly lower than 1.029-±0.365 in tissue adjacent,and the difference was statistically significant (t=3.129,P=0.000).The expression of miR-145 in patients whose biggest tumor size ≥ 1 cm was lower than patients whose biggest tumor size ＜1 cm.Compared with patients with single tumor,the expression of miR-145 in patients with multiple tumor was lower,and the dif ferences were statistically significant (P＜0.05).miR-145 expression was enhanced by miR-145 analogue.Compared with negative control,the proliferative ability of thyroid cancer cell TPC-1 was suppressed significantly,and the difference was statistically significant (P＜0.05).In addition,up-regulation of miR-145 expression could block thyroid cancer TPC-1 in G2/M phase.The apoptosis rate of thyroid cancer cell TPC-1 increased significantly (P＜0.05).Conclusions miR-145 expression is decreased significantly in PTC tissue,and is associated with clinical pathological features.Up-regulation of miR-145 expression can inhibit thyroid cancer cell proliferation,block the cell cycle,and promote apoptosis,miR-145 may play an important role in occurrence and development of thyroid cancer.
