Protective effects of pretreatment with ifenprodil on learning, memory and cognitive dysfunction induced by sevoflurane in neonatal rats
- VernacularTitle:艾芬地尔预处理对七氟醚导致的幼年大鼠学习记忆和认知能力损伤的保护作用及机制
- Author:
Haitao TIAN
;
Ping TIAN
;
Baojuan ZHANG
;
Yuelan WANG
;
Chengwei SONG
;
Haiou LI
- Keywords:
Sevoflurane;
NR2B;
Cognitive dysfunction;
Long-term potentiation
- From:
The Journal of Clinical Anesthesiology
2017;33(7):705-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role and mechanism of ifenprodil, which is the selective antagonist of N-methyl-D-aspartic acid subtype receptor NR2B, in soflurane-induced cognitive dysfunction in neonatal rats.Methods Twenty-eight 7-day-old Sprague Dawley rats, weighing 15-18 g, were randomly divided into 4 groups (n=7 each): control group (group C), ifenprodil group (group I), sevoflurane group (group S) and ifenprodil+sevoflurane group (group IS).Normal saline 0.2 ml was injected intraperitoneally in group C.Specific NR2B receptor antagonist ifenprodil 5 mg/kg was injected intraperitoneally at the corresponding time points in group I.Normal saline 0.2 ml was injected intraperitoneally and 2.0% sevoflurane was inhaled for 4 h in group S.Ifenprodil 5 mg/kg was injected intraperitoneally 2 h before sevoflurance inhalation, and 2.0% sevoflurance was inhaled for 4 h in group IS.The rats were then sacrificed 3 weeks after administration, their brains were immediately removed and hippocampal slices were prepared for electrophyisological experiments.The value of population spike amplitude (PSA) and long-term potentiation (LTP) were measured every 10 minutes.Induced LTP was recorded.Results Compared with group C, the values of PSA and rates of induced LTP were significantly decreased in group S (P＜0.01).The values of PSA and rates of induced LTP were significantly increased in group IS than those in group S (P＜0.01).Conclusion NR2B receptor is involved in sevoflurance-induced cognitive dysfunction in the neonatal rats.Pretreatment with ifenprodil 5.0 mg/kg can improve the neurotoxicity and protect the brain.
