IL-21 modulates biological characteristics of NK92 cells by upregulating SENP1 expression
10.7644/j.issn.1674-9960.2017.06.003
- VernacularTitle:IL-21通过 SENP1调控 NK细胞生物学特性
- Author:
Yuxiang LI
;
Qinqin XU
;
Huiyan SUN
;
Jing WEI
;
Xiaoyan ZHANG
;
Qinghua JIA
;
Fengjun XIAO
;
Lisheng WANG
- Keywords:
receptors;
interleukin-21;
small ubiquitin-related modifier proteins;
killer cells;
natural;
cell proliferation;
apoptosis
- From:Military Medical Sciences
2017;41(6):419-423,429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of SENP1 in regulation of biological characteristics of NK cells.Methods Lentivirus-mediated-Senp1-small-hairpinRNA (shRNA) transduction was applied to NK92 cells.The expression of SENP1 in NK92 cells was determined by real-time PCR and Western blot.The proliferation of NK92 cells was detected by CCK-8 assay.The apoptosis of NK92 cells was determined by Annexin Ⅴ and PI labeling.The cytotoxicity of NK92 cells against K562 cells was evaluated by luciferase reporter assay.Results Treatment of NK92 cells with IL-21 resulted in SENP1 upregulation.Lentivirus mediated SENP1 knockdown reduced proliferation and increased apoptosis in NK-92 cells,but SENP1 inhibition had slight impact on the cytotoxic ability of NK92 cells to kill K562 cells.Conclusion SENP1 mediates the regulatory effect of IL-21 on the proliferation and survival of NK92 cells.
