Pathogen isolation and whole-genome sequence analysis of human adenovirus type 55 outbreak on plateaus
10.7644/j.issn.1674-9960.2017.06.010
- VernacularTitle:高原地区55型腺病毒的病原分离及全基因序列分析
- Author:
Wenbo WANG
;
Yuan LIU
;
Yifan ZHOU
;
Liangqi GU
;
Xuelian ZHANG
;
Lin ZHANG
;
Maomao CHEN
;
Xianjun YANG
;
Ziying ZOU
;
Ping GUO
;
Wei QIU
;
Xiaobing HU
;
Quanshui FAN
- Keywords:
plateau;
adenovirus;
HAdV-B55;
pathogen isolation;
whole-genome sequencing
- From:
Military Medical Sciences
2017;41(6):453-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variation characteristics of adenovirus type 55 (HAdV-B55) gene on plateaus.Methods Throat swabs were collected from HAdV-B55 infected patients and used for virus isolation in HEp-2 cells.The whole-genome sequence was obtained by PCR and sequencing.HAdV-B55 gene sequence was blast with the previously reported virus.Results HAdV-B55 strains were isolated from throat swabs, which were named LS89/Tibet/2016.The whole-genome sequence was obtained and submitted to GenBank with the accession number of KY002683.No large fragment gene recombination was found between this HAdV-B55 strain and previous strains, and the sequence similarity with QS-DLL strain was 99.9%.Conclusion This study provides more information for the evolution patterns of adenovirus 55 and will contribute to the prevention and control of HAdV-B55 infection in the future.