Effect of tyrosine metabolites on cell proliferation,cell cycle and chemosensitivity of lung cancer cells
10.7644/j.issn.1674-9960.2017.06.018
- VernacularTitle:酪氨酸代谢物对肺癌细胞增殖、周期及化疗药物敏感性的影响
- Author:
Jie LI
;
Ke ZHAO
;
Xiangzhen KONG
;
Yiqun ZHAN
;
Xiaoming YANG
;
Changyan LI
- Keywords:
lung neoplasm;
tyrosine metabolites;
cell cycle;
cell proliferation;
cell apoptosis;
drug sensitivity
- From:Military Medical Sciences
2017;41(6):487-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of four tyrosine metabolites on the proliferation, cell cycle and chemosensitivity of lung cancer cells.Methods A549 cells were cultured with different concentrations of tyrosine metabolites:tyrosine, 4-hydroxyphenylpyruvate(HPPA), homogentisic acid(HGA) and fumaric acid (FA) in vitro.High content imaging analysis system was used to monitor cell proliferation.Flow cytometry was used to detect cell cycle and apoptosis.Protein and mRNA level of Bax and Bcl-2 were measured by Western blot.Results Tyrosine metabolites had no effect on cell cycle and proliferation of A549 cells.HGA decreased the cell apoptosis of A549 cells induced by Gefitinib with depressed Bax expression and elevated Bcl-2 expression.However, FA increased the apoptosis of A549 cells induced by Gefitinib with up-regulation of Bax and down-regulation of Bcl-2.No obvious effect of tyrosine and HPPA was detected on Gefitinib induced cell apoptosis.Conclusion HGA and FA may play an important role in drug sensitivity of lung cancer cells, indicating a critical role of tyrosine metabolism in lung cancer.
