Effect of mitomycin C in reducing hypertrophic scar in rat traumatic osteomyelitis model
10.3760/cma.j.issn.1001-8050.2017.07.014
- VernacularTitle:丝裂霉素C抑制大鼠创伤性骨感染软组织瘢痕形成的效果
- Author:
Peng WANG
;
Zhaoyan GONG
;
Chunhao SONG
;
Bo LIU
;
Cong CHEN
;
Wei LI
;
Dezhen YIN
;
Xiaotang XIN
;
Kai PANG
;
Peng XU
;
Weidong MU
- Keywords:
Osteomyelitis,traumatic;
Cicatrix;
Mitomycins
- From:
Chinese Journal of Trauma
2017;33(7):651-657
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of mitomycin C in reducing hypertrophic scar in rat traumatic osteomyelitis model.Methods A total of 120 Wistar rats were divided into control group (Group A,n =40),traumatic osteomyelitis group (Group B,n =40),traumatic osteomyelitis treated with Mitomycin C group (Group C,n =40),according to the random number table.The model of traumatic osteomyelitis was produced by Staphylococcus aureus.Muscle tissues around the focus were harvested at 15 d and 30 d postinjury.HE staining was used to observe the changes of muscle tissue structure.Immunohistochemistry was used to detect expression of transforming growth factor (TGF)-β1.Masson staining was used for collagen deposition evaluation.Western blot was used for detection of levels of TGF-β1 and collagen Ⅰ.Results HE staining revealed consistent alignment of fibers within the muscle in Group A.Fibrosis with the muscle was observed in both Group B and C,but the degree of muscle fiber disorder was decreased in Group C compared to Group B.Either 15 d or β0 d after injury,expressions intensity of TGF-β1,collagen fraction volume,and activation levels of TGF-β1 as well as collagen Ⅰ were higher in Group B and C than Group A,and all parameters were decreased in Group C compared to Group B (all P ＜ 0.05).Conclusion Mitomycin C can reduce hypertrophic scar formation in traumatic osteomyelitis model,and the potential mechanism relates to downregulated TGF-β1 and collagen Ⅰ.
