Establishment of HPLC fingerprints of Cajanus cajan leaves and determination of two constituents

VernacularTitle:木豆叶HPLC指纹图谱建立及2种成分测定
Author: Aili XU ; Xiaoli BI ; Sumei LI ; Yangxue LI ; Jieyi JIANG ; Buming LIU
Keywords: Cajanus cajan (L.) Millsp.; leaves; HPLC fingerprints; orientoside; luteolin
From: Chinese Traditional Patent Medicine 2017;39(7):1435-1439
CountryChina
Language:Chinese
Abstract: AIM To establish the HPLC fingerprints of Cajanus cajan (L.) Millsp.leaves and to determine the contents of orientoside and luteolin.METHODS The analysis of 65％ methanol extract from C.cajan leaves was performed on a 25 ℃ thermostatic Agilent Zorbax SB-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-1％ acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS There were twenty-one common peaks in ten batches of samples (S1-S10),whose similarities were more than 0.950,except for that of S3 (0.516).Orientoside and luteolin showed good linear relationships within the ranges of 0.089 5-3.960 μg and 0.015 5-0.408 μg,whose average recoveries were 99.43％ (RSD =1.32％) and 98.50％ (RSD =0.82％),respectively.The contents of two constituents in the samples from three growing areas (Guangdong,Yunnan and Hainan) showed obvious differences.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of C.cajan leaves.