Preparation and evaluation of fluorescent carbon dots (CDs) and CDs based immunofluorescence probe
10.7687/j.issn1003-8868.2017.06.022
- VernacularTitle:氨基化荧光碳点制备及其荧光探针构建
- Author:
Chunlin ZHANG
;
Yong ZHANG
;
Wangyuan CHE
;
Fan LI
;
Yuhai FANG
;
Zhifei HONG
;
Changjun LIU
- Keywords:
carbon dots;
fluorescence;
immunofluorescence probe;
nanomaterial;
E.coli O157:H7
- From:
Chinese Medical Equipment Journal
2017;38(6):22-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To fabricate an immunofluorescence probe system of carbon dots conjugated antibody based on antigen-antibody reaction principles.Methods A green one-step microwave assisted pyrolysis method was applied to preparing fluorescent carbon dots (CDs) using aminoglucose as carbon source and the obtained CDs were conjugated with antibody via EDC/NHS reactions to build CDs based fluorescent probe.Furthermore,the properties of CDs and CDs based probe system were evaluated by Fourier transform infrared (FTIR) spectra,transmission electron microscopy (TEM),UV-vis absorption and so on.Results The as-prepared CDs showed excellent fluorescence and hydrophilicity and CDs based immunofluorescence probe exhibited the capability of quick detection of E.coli O157:H7.Cinclusion Fluorescent CDs as one new emerging environment-friendly nanomaterial has great potential in biosensors.
