MiR-22-3p suppresses cell proliferation by targeting AEG-1 in NSCLC
10.3969/j.issn.1006-5725.2017.14.005
- VernacularTitle:miR-22-3p靶向AEG-1抑制非小细胞肺癌细胞增殖
- Author:
Yongmei CHANG
;
Wensen YAN
;
Xiaoyun JIANG
;
Cong SUN
;
Qingfeng LIU
;
Jun WANG
;
Mingzhi WANG
- Keywords:
NSCLC;
miR-22-3p;
AEG-1;
proliferation
- From:
The Journal of Practical Medicine
2017;33(14):2267-2271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and effect of miR-22-3p in non-small cell lung cancer (NSCLC). Methods The miR-22-3p expression level in seventy-six NSCLC tissues and para-cancer tissues was detected by qRT-PCR. The relationship between the expression of miR-22-3p and gender,age,tumor size,histolo-gy grade,pathological type and lymph node metastasis was analyzed. The function of miR-22-3p on the prolifera-tion of NSCLC cells was tested by growth curve assay. Target genes of miR-22-3p were predicted by online software Targetscan. Luciferase reporter assay and qRT-PCR was used to certificate the prediction. Results The expression of miR-22-3p was increased in NSCLC tissues than the para-cancer tissues and was correlated to lymph node metas-tasis. Overexpression of miR-22-3p could suppress the proliferation of A549 cells. Astrocyte-Elevated Gene-1(AEG-1) was predicted to be a target of miR-22-3p. MiR-22-3p was revealed to bind to AEG-13′UTR by luciferase report-er assay. Overexpression of miR-22-3p could inhibit the expression of AEG-1 in A549 cells. Suppression of miR-22-3p could increase AEG-1 expression. Conclusion MiR-22-3p could inhibit the proliferation of NSCLC by tar-geting AEG-1.
