Mechanism of fatty liver induced by high-fructose-feeding in rats and intervention effect of fenofibrate
10.3969/j.issn.1006-5725.2017.14.009
- VernacularTitle:高果糖喂养大鼠致脂肪肝的机制及非诺贝特的干预作用
- Author:
Yang LI
;
Zhe GAO
;
Luping REN
;
Pu ZHANG
;
Guangyao SONG
- Keywords:
fatty liver;
fenofibrate;
fatty acid synthase;
autophagy;
endoplasmic reticulum stress
- From:
The Journal of Practical Medicine
2017;33(14):2287-2290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of fenofibrate intervention on high-fructose-feeding-induced liver steatosis in rats and explore the possible mechanism. Methods Male Wistar rats were randomly divided into control group ,high fructose group and fenofibrate group[fenofibrate intervention started after 8 weeks of high fructose feeding ,30 mg/(kg · d)]. Rats were sacrificed after 12-week of high fructose feeding. Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),free triglyceride(TG)and liver TG content were determined;protein levels of fatty acid synthase(FAS),endoplasmic reticulum stress mark-er Bip and autophagy markers such as Atg7,Beclin1,LC3 and the related pathway mTOR in liver tissues were de-tected. Results Compared with those in control group and fenofibrate group,serum AST,serum total cholesterol, blood free TG and hepatic TG were significantly increased in high-fructose group(P < 0.01). The protein expres-sion of Fas,Bip and mTOR were significantly increased in high-fructose group compared with those in control group and fenofibrate group;the protein expression of Atg7,beclin1 and LC3 were significantly decreased in high-fructose group compared with those in control group and fenofibrate group. Conclusions Long-term high-fructose-feeding induces fatty liver and liver cell injury ,and may affect ERS and autophagy. High-fructose-feeding-in-duced fatty liver may be improved by fenofibrate and its underlying mechanism might be associated with Fas,ERS and autophagy in liver.
