Treatment effect of 1,25(OH)2D3 on liver fibrosis by inhibiting HSC activation and regulating Th cell dif-ferentiation
10.3969/j.issn.1006-5725.2017.13.008
- VernacularTitle:1,25(OH)2D3抑制肝星状细胞激活和调控Th细胞分化治疗肝纤维化
- Author:
Chunjie XU
;
Lei GU
;
Chunhui JIANG
;
Longci SUN
;
Ye LIU
;
Hong ZHOU
;
Qing XU
- Keywords:
liver fibrosis;
1;
25(OH)2D3;
Th cell;
Th17
- From:
The Journal of Practical Medicine
2017;33(13):2100-2104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of 1-alpha,25-dihydroxy-cholecalcifero(1,25(OH)2D3)on liver fibrosis and its mechanism. Methods Degree of liver fibrosis was assessed through pathological detection and blood biochemical examination of liver function. Immunohistochemical assay was used to detect expressions of α-SMA,TGF-βand collagen I to observe activation level of hepatic stellate cells. Impact of 1,25(OH)2D3 on CD4+T cell differentiation was analyzed by flow cytometry,ELISA,and RT-PCR. Results 1,25(OH)2D3 improved the structure of the liver tissue and liver fibrosis. Expressions of collagen I ,TGF-βandα-SMA were significantly ele-vated in the liver tissue in rats with fibrosis(P < 0.05)but were markedly decreased after treatment with 1,25 (OH)2D3(P < 0.05). After 1,25(OH)2D3 treatment,the proportion of Th17 cells reduced while that of Th2 in-creased;concentration levels of IFNγ,IL-17A,and IL-22 markedly declined but IL-4 elevated(P>0.01);and ex-pressions of RORγt and T-bet decreased whereas GATA3 expression increased(P>0.01);as compared with those in the control group. Conclusions 1,25(OH)2D3 can alleviate the degree of liver tissue by lowering HSC activation and regulating Th cell differentiation.
