Effect of FABP4 DNA methylation on abnormal lipid metabolism in trophocyte treated with L-NAME
10.3969/j.issn.1006-5725.2017.14.002
- VernacularTitle:脂肪酸结合蛋白4 DNA甲基化在L-NAME干预胎盘滋养细胞脂代谢异常中的作用
- Author:
Jun YANG
;
Caiyan MAO
;
Songhao YANG
;
Anning YANG
;
Mei DENG
;
Kai WU
;
Xiaoling YANG
;
Yideng JIANG
;
Huiping ZHANG
- Keywords:
pregnancy-induced hypertension syndrome;
trophoblastic cell;
FABP4;
DNA methyla-tion;
lipid metabolism
- From:
The Journal of Practical Medicine
2017;33(14):2255-2258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fatty acid binding protein 4(FABP4)DNA methylation on abnormal lipid metabolism in placental trophoblastic dyslipidemia. Methods Human placental trophoblast cell line(HTR-8)was treated with L-NAME of 100 μmol/L for 48 h. The lipid content in placental trophoblasts was detected by chemical enzyme-colorimetry. The FABP4 DNA methylation level in placenta trophoblasts was detected by nested-touch down methylation specific PCR (NT-MSP). the mRNA and protein expression of DNMT1 and FABP4 were detected by qRT-PCR and Western Blot,respectively,in trophoblast cells. Results The lipid content in trophoblasts significantly increased as compared with the control(P < 0.05). Expression of FABP4 mRNA and protein increased(P < 0.05),while FABP4 methylation level and expression of DNMT1 significantly decreased (P<0.05)after treatment with L-NAME. Conclusions FABP4 DNA methylation is involved in the regulation of lipid metabolism in placental trophoblastic cells of hypertensive disorder complicating pregnancy.
