Effects of PTEN and its mutants on AKT phosphorylation in gastric cancer cells
10.3969/j.issn.1006-5725.2017.14.003
- VernacularTitle:PTEN及其突变体对胃癌细胞AKT磷酸化的影响
- Author:
Hailan ZHONG
;
Guiliang WANG
;
Jianbo WEN
- Keywords:
stomach neoplasms;
PTEN;
AKT phosphorylation;
insulin;
rhEGF
- From:
The Journal of Practical Medicine
2017;33(14):2259-2262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of PTEN and missense mutations in PTEN phosphotase domain on AKT phosphorylation in AGS and BGC-823 cells. Methods The plasmids of wtPTEN,PTEN-C124S which PTEN mutant is in both lipid and protein phosphotase domain and PTEN-G129E which PTEN mutant is only in lipid phosphotase domain were respectively transfected into AGS and BGC-823 cells. The cells were stimulated with insulin or rhEGF after serum starvation overnight. The levels of AKT phosphorylation were detected by Western blot. Results Both insulin and rhEGF can activate AKT phosphorylation in gastric cancer cells. Overexpressed PTEN inhibitedAKT phosphorylation induced by insulin or rhEGF(P < 0.05). PTEN mutants C124S or G129E could not inhibitAKT phosphorylation(P > 0.05). Conclusions PTEN can inhibit AKT phosphorylation induced by insulin or rhEGF in gastric cancer cells. Missense mutations in the 124th or 129th amino acid of PTEN phospho-tase domain do not exert inhibitive function.
