The effect of salinomycin on cancer stem cell formation of prostate cancer cell line DU145 through mTOR signaling pathway
10.3969/j.issn.1006-5725.2017.13.006
- VernacularTitle:盐霉素及mTOR信号通路对前列腺癌DU145干细胞的影响
- Author:
Hongtao JIANG
;
Tao WU
;
Yunsheng ZHANG
;
Luogen LIU
;
Maolin JIANG
;
Lian PENG
;
Hong ZHANG
;
Mingshu ZHOU
;
Yi WANG
- Keywords:
salinomycin;
cancer stem cells;
aldehyde dehydrogenase;
mTOR signaling pathway
- From:
The Journal of Practical Medicine
2017;33(13):2092-2096
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of salinomycin on cancer stem cell formation of prostate cancer cell line DU145 and its possible mechanisms,providing theoretical basis for the clinical application of salino-mycin. Methods (1)DU145 cells were treated with salinomycin. The percentage of ALDH+cells,which was used as the marker of cancer stem cells,was detected by flow cytometry.(2)After treated with salmonin,DU145 cells were subjected to Western-Blot analysis for the expression of mTORsignal pathway-related proteins such as p-70s6k, p-p70s6,p-s6 and so on. 3)DU145 cells were treated with salinomycin combined with mTOR signal pathway inhibi-tor rapamycin,and the ALDH+cancer stem cells were detected using flow cytometer. Results (1)Salmonomycin significantly inhibited ALDH-positive cancer stem cells in DU145cell line(inhibition rate in 77.8%),which was twice as high as that of traditional anticancer drug paclitaxel(which has a inhibition rate of 38.64%). This results suggesting that salinomycin would have the effect of inhibiting cancer stem cells. (2)The expression ofm-TOR p-70s6k,p-p70s6 and p-s6 in mTOR signaling pathway was inhibited by salinomycin in a time-dependent and dose-dependent manner,suggesting that salinomycin would inhibite mTOR signaling pathway.(3)Salinomycin combined with rapamycin can decrease the proportion of ALDH-positive DU145 cancer stem cells(inhibition rate in 77.95%), suggesting that salinomycin may inhibit ALDH-positive DU145 stem cells through the mTOR signaling pathway. Conclusion Salinomycin may play an important role in inhibiting cancer stem cells by inhibiting mTOR pathway signaling.
