Effect of cyr61 on proliferation and differentiation of hepatic progenitor cells
10.3969/j.issn.1006-5725.2017.13.010
- VernacularTitle:富半胱氨酸蛋白61在肝卵圆细胞增殖和分化中的作用及意义
- Author:
Wei DING
;
Hongbo TAN
;
Xuemei LI
;
Changli JIANG
;
Dixin CAI
;
Yue ZHANG
;
Xinghua PAN
- Keywords:
CCN/Cyr61;
β-catenin;
HOCs;
2-AAF
- From:
The Journal of Practical Medicine
2017;33(13):2109-2112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regeneration and differentiation of HOCs in the 2-AAF/PHx rat models. To explore the expression of Cyr61and its mechanism in differentiation of HOCs in vitro. Methods In 2-AAF/PHx rats model,induction and expansion of HOCs were detected by immunochemistry and HE staining. West-ern blot was used for observing the expression of Cyr61. Furthermore,the expression of Cyr61 andβ-catenin were detected by Western blot in differentiation of WB-F344 cells in vitro. Results Cyr61 protein level increased as a re-sult of HOCs in rats livers after 2-AAF/PHx. In addition,the expression of Cyr61 and β-catenin significantly in-creased during WB-F344 cells differentiation in vitro. Conclusions Cyr61 might play an important role as a signal-ing mediator in HOCs response and closely correlate with Cyr61 andβ-catenin in proliferation and differentiation of HOCs.
