Octreotide against liver injury by alleviating endoplasmic reticulum stress in rats with hepatic fibrosis
10.3969/j.issn.1006-5725.2017.13.011
- VernacularTitle:奥曲肽通过抑制内质网应激减轻肝纤维化大鼠肝损伤
- Author:
Huiping ZHANG
;
Chao ZHANG
;
Fangyue LI
;
Ran AN
;
Chong ZHANG
- Keywords:
liver injury;
octreotide;
hepatic fibrosis;
endoplasmic reticulum
- From:
The Journal of Practical Medicine
2017;33(13):2113-2116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of octreotide(OCT)on plasma ALT and AST and hepatic tissue protein expression of Bcl-2,Bax,GRP78 and CHOP in rats liver with fibrosis,to explore whether it relieves liver injury in rats with hepatic fibrosis by relieving endoplasmic reticulum stress(ERS)reaction. Methods For-ty Sprague-Dawley rats were randomly divided into Con group ,Mod group and OCT-treatment groups at high and low doses(1,10 mg/kg/12 h respectively). Except for rats in Con group,all rats were injected with 40%CCl4 sub-cutaneously twice a week for 8 weeks to establish hepatic fibrosis ,and those in OCT-treatment groups were injected at different doses of OCT twice a day at the same time. Plasma ALT and AST was assayed;the isolated livers were evaluated for histopathological changes by HE staining and Masson staining;hepatic histological grading and stag-ing were assessed by Ishak scoring system;Bcl-2,Bax,GRP78 and CHOP protein expression levels were deter-mined by Western blot. Results Compared with those in Con group ,the degrees of inflammatory cell infiltration and fibers,hepatic histological grading and staging,plasma ALT,AST activities and Bax,GRP78,CHOP protein levels were significantly increased in Mod and OCT-treatment groups ,but Bcl-2 protein level was significantly de-creased at the same time(P<0.01). Compared with those in Mod group ,the degrees of inflammatory cell infiltra-tion and fibers,hepatic histological grading and staging,plasma ALT,AST activities and Bax,GRP78,CHOP protein levels were significantly decreased in OCT-treatment groups ,but Bcl-2 protein level was significantly in-creased at the same time(P < 0.05). There was no significant difference between 2 OCT-treatment groups(P >0.05). Conclusions OCT can reduce the level of liver injury in rats with hepatic fibrosis ,thus the mechanism may be related to relieving the ERS reaction in liver.
