Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells
10.13481/j.1671-587x.20170415
- VernacularTitle:五倍子酸对人胃癌SGC-7901细胞迁移能力的影响
- Author:
Liping WANG
;
Ruo FENG
;
Liyang ZOU
;
Yi DING
- Keywords:
gallic acid;
SGC-7901 cells;
cell migration;
vascular endothelial growth factor
- From:
Journal of Jilin University(Medicine Edition)
2017;43(4):739-742,前插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of gallic acid (GA) on the migration of human gastric carcinoma SGC-7901 cells, and to explore its mechanism.Methods: The human gastric carcinoma SGC-7901 cells were cultured and divided into control group and 3.125, 6.250, 12.500, 25.000, 50.000,100.000 mg·L-1 GA groups. The inhibitory rates of proliferation of SGC-7901 cells in various groups were examined by MTT assay;the migration abilities of SGC-7901 cells in various groups were measured with scratch assay;the expression levels of vascular of endothelial growth factor (VEGF) in various groups were detected by immunocytochemistry.Results: Compared with control group, the inhibitory rates of proliferation of SGC-7901 cells in different doses of GA groups were significantly increased in a dose-dependent manner(F=59.451,P<0.01).Compared with control group, the wound healing rates in different doses of GA groups were significantly decreased (P<0.01).Compared with control group, the expression levels of VEGF protein in 12.500 and 25.000 mg· L-1 GA groups were decreased (P<0.05).Conclusion: GA could inhibit the proliferation and migration of SGC-7901 cells through down-regulating the expression levels of VEGF protein.
