Differential expressions of ciaH, eno and pykF genes of fluoride-resistant Streptococcus mutans cultivated in fluoride environment and its significance
10.13481/j.1671-587x.20170417
- VernacularTitle:氟环境下变形链球菌耐氟菌株ciaH、eno和pykF基因的差异表达及其意义
- Author:
Qi ZHANG
;
Hongyan ZHAO
;
Zhimin ZHANG
;
Hong ZHANG
;
Wenyue LI
;
Bo CHAO
;
Yaoyao YANG
- Keywords:
ciaH;
eno;
pykF;
Streptococcus mutans;
fluoride-resistant Streptococcus mutans;
fluoride resistance
- From:
Journal of Jilin University(Medicine Edition)
2017;43(4):747-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between ciaH, eno, pykF genes and fluoride resistance through determining the differential expressions of ciaH, eno and pykF genes of fluoride-resistant Streptococcus mutans cultivated in fluoride environment. Methods:The cultured Streptococcus mutans and their fluoride-resistant strains were divided into UA (Streptococcus mutans subcultured in BHI without NaF), FR (fluoride-resistant Streptococcus mutans subcultured in BHI without NaF) and FFR (fluoride-resistant Streptococcus mutans subcultured in BHI containing 1 g·L-1 NaF) groups.After 11 h (logarithmic phase) and 20 h (platform stage) cultivation, the expression levels of ciaH, eno and pykF mRNA were detected by RT-PCR method.Results:Compared with FR group, the expression levels of ciaH, eno and pykF mRNA in FFR group were increased both in the logarithmic phase and the platform stage(P<0.05 or P<0.01).Compared with UA group, the expression levels of eno and pykF mRNA in FR group were decreased both in the logarithmic phase and the platform stage(P<0.01), whereas the expression level of ciaH mRNA had no significant difference in the logarithmic phase (P>0.05), but it was increased in the platform stage (P<0.01).Conclusion:Fluoride can increase the expression levels of ciaH, eno and pykF genes in fluoride-resistant Streptococcus mutans, indicating that these genes are related to the production of fluoride resistance.
