Evaluation of HCV-IgG,HCV-cAg and HCV-RNA in the diagnosis of Hepatitis C
10.3969/j.issn.1673-4130.2017.14.007
- VernacularTitle:HCV-IgG、HCV-cAg与HCV-RNA对丙型肝炎诊断的评价
- Author:
Hong WU
;
Yong XU
;
Jinhong ZHU
;
Xiaoman WU
;
Xiaoyan DENG
- Keywords:
hepatitis C virus core antigen;
hepatitis C virus;
hepatitis C virus;
hepatitis C
- From:
International Journal of Laboratory Medicine
2017;38(14):1890-1891,1894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of hepatitis C virus core antigen(HCV-cAg),hepatitis C virus(HCV-IgG) and hepatitis C virus(HCV-RNA) in the laboratory diagnosis of Hepatitis C.Methods HCV-cAg and HCV-IgG were detected by enzyme-linked immunosorbent assay(ELISA),HCV-RNA was detected by real-time fluorescent quantitative polymerase chain reaction(RT-PCR) in 84 suspected HCV patients and 87 healthy control subjects.Results In 84 suspected HCV patients,the HCV-IgG positive rate was 84.5%,HCV-cAg positive rate was 13.1%,HCV-RNA positive rate was 52.4%.Among 71 cases of HCV-IgG positive patients,there were 35 cases with negative HCV-RNA,the false positive rate was 49.3%.In 11 cases of HCV-cAg positive patients,there were 5 cases with negative HCV-RNA,the false positive rate was 45.5%.In 44 cases of HCV-RNA positive diagnosis of hepatitis C patients,HCV-IgG false negative rate was 18.2%,HCV-cAg false negative rate was 86.4%.The false negative rate of combined detection of HCV-cAg and HCV-IgG was 13.6%,and the true positive rate was 100.0%.Conclusion HCV-cAg and HCV-IgG have certain false negative and false positive in laboratory diagnosis of HCV,combine these two methods,or joint with HCV-RNA detection,could reduce the rate of missed diagnosis.
