Establishment of a direct detection method for human platelet antigen1-17,Cab allele whole blood
10.3969/j.issn.1673-4130.2017.14.009
- VernacularTitle:1种人类血小板抗原1~17及Cab等位基因全血直接检测方法的建立
- Author:
Yipeng ZHANG
;
Zhiping HUANG
;
Dongmei NIE
;
Wangchun ZHENG
;
Weidong LIU
- Keywords:
human platelet antigen;
Cab;
subtype;
polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2017;38(14):1895-1897,1900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an acurate and convenient method to distinguish human platelet antigen(HPA) SNPs based on Target Enriched Multiplex-PCR(TEM-PCR),fluorescent probe melting curve analysis and blood direct PCR.Methods Design TEM-PCR primers and probes of HPA1-17,Cab alleles,amplify target sequences of all 18 alleles by blood direct PCR and distinguish different SNPs by melting curve of probes.Results The TEM-PCR could amplify all target sequences of 18 alleles and the melting curve analysis could distinguish those SNPs,the accuracy was equal to PCR-SSP method and the process was more convenient without blood genomic DNA extraction and subsequent gel electrophoresis thus decrease the cross-contamination risk.Conclusion Successfully established a HPA1-17,Cab alleles distinguishing method based on TEM-PCR,blood direct PCR and fluorescent probe melting curve analysis technique.
