The expression and significance of Toll-like receptors-4 in peripheral blood and placental tissuein gestational diabetes mellitus patients
10.3969/j.issn.1673-4130.2017.14.011
- VernacularTitle:Toll样受体4在妊娠期糖尿病患者外周血和胎盘组织中的表达及意义
- Author:
Qin XIE
;
Yanhua JIANG
;
Hongli HUANG
- Keywords:
Toll-like receptors-4;
gestational diabetes mellitus;
peripheral blood;
placental tissue
- From:
International Journal of Laboratory Medicine
2017;38(14):1901-1903,1906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of Toll-like receptors-4 in peripheral blood and placental tissue in gestational diabetes mellitus (GDM) patients.Methods From February 2013 to February 2015.a total of 30 cases of gestational diabetes mellitus patients(GDM group) and 30 cases of normal pregnant people(health group)were selected as research objects.Peripheral blood and placental tissue of two groups were collected.The expression of TLR4 mRNA in peripheral blood mononuclear cells was detected by RT-PCR,the expression of TLR4 protein in placenta tissue was detected by immunohistochemistry.Results The expression of TLR4 mRNA in peripheral blood mononuclear cells of the observation group was significantly higher than that of the health group[(0.63±0.12) vs.(0.32±0.07)],the difference was statistically significant(t=12.223,P<0.05).The expression of TLR4 in villous trophoblast cells,decidual cells and amniotic epithelial cells in GDM group was significantly higher than that in the health group(Z=2.325,2.374,2.162,P<0.05).Conclusion The expression of TLR4 in peripheral blood mononuclear cells,villous trophoblast cells and decidual cells in gestational diabetes mellitus patients significantly enhanced,suggesting that TLR4 might be related to the e gestational diabetes mellitus.
