To explore the preparation method of different animal erythrocytehemolysin in experimental teaching of medical immunology
10.3969/j.issn.1673-4130.2017.14.018
- VernacularTitle:不同动物红细胞制备实验教学用溶血素的方法探讨
- Author:
Erhua LI
;
Jiyun YE
;
Hao ZOU
;
Fang CHEN
;
Yunxia LIU
;
Caijun ZHANG
- Keywords:
hemolysin;
red blood cell antigen;
titer;
immunological;
experimental teaching
- From:
International Journal of Laboratory Medicine
2017;38(14):1921-1924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect different animal erythrocyte hemolysins titer,and compare the application of these hemolysins in immunological experimental teaching,for selecting the better method of preparing high titer hemolysin for experimental teaching of medical immunology.Methods A total of 40 experiment rabbits were divided into 4 groups in this study,and immunized by sheep red blood cell(SRBC) and porcine red blood cell(PRBC) through different immunization procedures to prepare the hemolysin,detect and compare these 4 groups hemolysins titer by the complement hemolysis test.Results Rabbit Anti-SRBC in the group A was 1∶4 800,rabbit Anti-PRBC in the group B was 1∶1 200,rabbit Anti-SRBC in the group C was 1∶1 000,rabbit Anti-PRBC in the group D was 1∶200.Conclusion The hemolysin titer of the rabbit Anti-PRBC was lower than that of the rabbit Anti-SRBC by the same immunization procedures,and the immunization procedure by intradermal multi-point and auricular vein injection is the better method of preparing high titer hemolysin,so PRBC could replace SRBC as antigen,and immunize the rabbits for preparing hemolysin,which could be used in experimental teaching of medical immunology.
