Quantitative detection of the loop mediated isothermal amplification gene in the loopmediated isothermal amplification of Japanese encephalitis and dengue fever
10.3969/j.issn.1673-4130.2017.14.021
- VernacularTitle:乙型脑炎和登革热病毒的环介导等温扩增基因定量检测技术研究
- Author:
Yang ZHANG
;
Chong ZHOU
;
Tao SUN
;
Haihua YI
- Keywords:
Japanese encephalitis;
dengue fever;
loop-mediated isothermal amplification;
gene quantitative;
rapid detection
- From:
International Journal of Laboratory Medicine
2017;38(14):1931-1934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a loop-mediated isothermal amplification(LAMP) quantitative method for rapid detection of Japanese encephalitis and dengue fever virus.Methods According to the LAMP principle,design primers for LAMP detection and reaction system,establish LAMP detection method,and to evaluate the linear relationship between initial copy number and the specificity,sensitivity,repeatability and the reaction time(fluorescence signal value of 1×104 corresponding time).Results One sets of LAMP primers could be used to complete the detection work in 0.5 h.The sensitivity of LAMP detection technology was 10 times higher than that of the classical PCR technology,and no cross reaction with other viruses,and the coefficient of variation of the average test was less than 5%.There was a good linear relationship between cycle threshold and template concentration.Conclusion This method has high specificity,sensitivity,simple operation,which is easy to get the results,low equipment requirements and rapid,suitable for primary health institutions and the field inspection agencies for wide applications.
