The value of quantitative PCR detection in early skin tissue fluid of syphilis
10.3969/j.issn.1673-4130.2017.14.025
- VernacularTitle:早期皮损组织液的定量PCR检测在梅毒诊断中的价值分析
- Author:
Liting WU
;
Wuying OU
;
Yiyun MA
- Keywords:
syphilis;
tissue fluid;
fluorescence quantitative polymerase chain reaction;
treponema pallidum
- From:
International Journal of Laboratory Medicine
2017;38(14):1942-1944
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) detection in early skin tissue fluid of syphilis.Methods A total of 40 patients of suspected syphilis who received therapy from September 2014 to September 2016 in our hospital were selected in this study.Five mL venous blood samples were collected in all the patients,and detected by toluidine red unheated serum test (TRUST) and treponema pallidum antibody enzyme-linked immunosorbent assay (ELISA),and skin tissue fluid were collected and performed FQ-PCR detection,all patients were treated with benzylpenicillin for 3 and 6 months,then detected again,the conversion rates were record.Results There was no significant difference in the detection rate of ELISA and FQ-PCR[97.50%(39/40) vs.95.00%(38/40),P>0.05].The total detection rate of ELISA and FQ-PCR were significantly higher than that of TRUST[97.50%(39/40) vs.67.50%(27/40),95.00%(38/40) vs.67.50%(27/40),P<0.05].After 3 and 6 months of treatment,negative rates of ELISA and FQ-PCR were significantly higher than that of TRUST(P<0.05),there was no significant difference on negative rate between FQ-PCR and ELISA (P>0.05).In the FQ-PCR detection results,the average value of TP-DNA was significantly decreased after treatment,there were significant differences in the phase Ⅰ,phase Ⅱ compared with before treatment(P<0.05).Conclusion FQ-PCR could be used to measure treponema pallidum (TP-DNA) effectively in early stage,it′s conducive to the diagnosis of syphilis,the clinical application value is high.
