Effect of long non-coding RNA ROR on proliferation and apoptosis of pancreatic cancer cells by regulating notch1 protein
10.3969/j.issn.1006-5725.2017.12.007
- VernacularTitle:长链非编码RNA ROR通过notch1蛋白调控胰腺癌细胞的增殖凋亡
- Author:
Bing LI
;
Zhengfang XIONG
;
Yamin. GUO
- Keywords:
Pancreatic cancer;
LncRNA;
Notch1 protein;
Apoptosis
- From:
The Journal of Practical Medicine
2017;33(12):1922-1927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of long chain noncoding RNA RORin regulating prolifer-ation and apoptosis of pancreatic cancer cell. Methods Pancreatic cancer cell line BxPC-3 was selected. The RNA level of lncRNA ROR and notch1 was detected by RT-PCR.Notch1 protein level was detected by Western blot. The regulating relationship between lncRNA ROR and notch1 was analyzedby RNAhybird and luciferase re-porter assay. At last ,CCK-8 and TUNEL were applied to detectthe proliferation and apoptosis of cell line. Re-sults lncRNA ROR and notch1 were highly expressed in pancreatic cancer tissue ,compared with normal tis-sues. There was positive correlation between them. lncRNA ROR was over-expressed in BxPC-3,cell proliferation activity was increased and the percentagesof DNA damaged positive cells was decreased ,accompanied by in-creased levels of notch1 mRNA and protein. Luciferase assay confirmed that ROR could bind to notch1and inhibit its activity by miR-137. Compared with control group ,the proliferation of pcDNA-ROR + si-notch1 cells reduced and the proportion of TUNEL positive cells increased. The differences were statistically significant. Conclusionl ncRNA ROR regulated the proliferation and apoptosis of pancreatic cancer cells by promoting the expression of notch1.
