The study on the construction of Pluronic P85 coated poly(butylcyanoacrylate) nanoparticles and its brain target effect on a rat model of mesial temporal lobe epilepsy
10.3969/j.issn.1002-0152.2017.06.008
- VernacularTitle:普朗尼克P85修饰的苯妥英钠纳米粒对颞叶内侧癫痫大鼠模型的脑靶向作用
- Author:
Ziyan FANG
;
Caifeng GUO
;
Fengchun WU
;
Jiaming QIN
;
Yuping NING
;
Liemin ZHOU
- Keywords:
Nanoparticles;
Temporal lobe epilepsy;
P-glycoprotein;
Phenytoin;
Targeting
- From:
Chinese Journal of Nervous and Mental Diseases
2017;43(6):356-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to evaluate that whether Pluronic P85 coated poly(butylcyanoacrylate) nanoparti-cles was able to deliver antiepileptic drug phenytoin into the brain va bypassing mesial temporal lobe epilepsy (MTLE)-induced Pgp in a rat model of MTLE. Methods The rat model of MTLE, induced by li-pilocarpine, was divided in-to two groups (6 for nanoparticle drug group and 7 for PHT drug group). Immunohistochemistry assay was performed to detect Pgp expression at the hippocampus. Nanoparticles were prepared by interfacial polymerization method. Dialysate samples of brain were collected at 30, 60, 120, 180, 240 and 300 min after drug administration by microdialysis tech-nology. Samples were analyzed by high performance liquid chromatography (HPLC). Results The area under the curve (AUC) ratio of brain/plasma in Nanoparticle drug group was 0.370.10 which was significantly higher compared with 0.190.06 in conventional PHT drug group (P<0.05). The Pgp immunopositive area, as assessed by analysis of labeled surface area, was higher in the DG, CA3 and CA1 sector in the hippocampus of MTLE rats when compared to the normal rats. Conclusions Pluronic P85 coated PBCA nanoparticles can significantly deliver PHT into brain via bypassing MTLE-induced Pgp in a rat model of MTLE.
