Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats
10.13481/j.1671-587x.20170418
- VernacularTitle:MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响
- Author:
Zhujun YANG
;
Xu HOU
;
Wenwen YU
;
Kuo YAN
;
Yuqin SHEN
;
Xinhua SUN
- Keywords:
MT01;
tooth movement;
toll like receptors 9;
tumor necrosis factor receptor associated factor 6;
interleukin-6;
polymerase chain reaction
- From:
Journal of Jilin University(Medicine Edition)
2017;43(4):752-756,前插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of expression levels of TLR9 and IL-6 in the periodontal tissue during the experimental tooth movement of the rats, and the effecst of the MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue,and to clarify its related mechanisms.Methods:Thirty Wistar rats were randomly divided into MT01 intervention group(n=6) and non-MT01 group(n=24).Force of 0.49 N was applied to move the upper first molars mesially. The rats in Non-MT01 intervention group were sacrificed on the days 3,7,14 and 21, and the rats in MT01 intervention group were all sacrificed on the day 7. RT-qPCR was used to detect the expression levels of TLR9,TRAF6 and IL-6 mRNA in maxillary first molar alveolar bone in each group.Results:The expression levels of IL-6 and TLR9 mRNA in loaded side were higher than those in control side(P<0.05), and reached the maximum levels on the day 7(P<0.01);with the interpose of MT01, the expression levels of TLR9 and TRAF6 mRNA were lower than control side(P<0.01).Conclusion: MT01 could down-regulate the expression levels of TLR9 and TRAF6 during orthodontic tooth movement and eventually resists the inflammation during the tooth movement.
