The research of the CENP-W in regulating invasion of gliomas

VernacularTitle:着丝粒蛋白W调控脑胶质瘤侵袭性的作用
Author: Jianbin LI ; Jinhua WAN ; Meng XU ; Haiyang LUO ; Qiankun JI ; Shigang LYV ; Minhua. YE
Keywords: CENP-W; RAS; Gliomas; Invasion
From: The Journal of Practical Medicine 2017;33(12):1953-1956
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of the CENP-W in gliomas and investigate the effects of its invasion. Methods The expression level of the CENP-W in gliomas with varied pathologic grade were detected by immunohistochemical analysis,RealTime PCR,and Western Blotting. U251 cells were transfected with the specific siRNA to repress the CENP-W expression level. The invasion ability of U251 cells were examined by Transwell Chamber assay ,while RAS mRNA and protein levels were detected at the same time. Results The expression levels of the CENP-W in glioma tissues were significantly high and the CENP-W gene could enhance the invasion of U251 cells . The expression of RAS was down-regulated when the expression of CENP-W was repressed. Conclusion The CENP-W has an oncogenic role in human brain gliomas and may regulate the invasion of gliomas by adjusting the RAS signaling pathways.