Comparison of detection value in the colloidal gold and the passive particle agglutination methods in myco-plasma pneumonia
10.3969/j.issn.1006-5725.2017.12.038
- VernacularTitle:胶体金法和被动凝集法在肺炎支原体检测中的比较
- Author:
Cuicui LI
;
Aiwen LI
;
Xia MIAO
;
Sujing SHEN
;
Nan. WANG
- Keywords:
Mycoplasma pneumonia;
Colloidal gold method;
Passive agglutination method
- From:
The Journal of Practical Medicine
2017;33(12):2036-2038
- CountryChina
- Language:Chinese
-
Abstract:
Objective Comparison the coincidence rate in the colloidal gold method and the passive agglu-tination method to detect mycoplasma pneumoniae (MP) infection, discuss the clinical value in rapid diagnosis of MP infection in the two methods. Methods Two-hundred patients with MP infection, including 100 cases in the the children group, and 100 cases in the adult group, were detected in MP-IgM antibody in serum with the colloidal gold method and the passive agglutination method. Results The positive rate of MP-IgM antibody with the passive agglutination method were slightly higher than that of the colloidal gold method in the children group (P > 0.05), While the positive rate of MP-IgM antibody with the passive agglutination method in the adult group were signifi-cantly higher than that of the colloidal gold method (P<0.05). When the antibody titer of MP-IgM antibody were 1:60, ≥1:320 in the children group, the coincidence rate of the positive results with the colloidal gold method and the passive agglutination method were 95.40%, 95.30%;When the antibody titer of MP-IgM antibody were 1:80, 1:160,≥1:320 in the adult group, the coincidence rate of the positive results with the colloidal gold method and the passive agglutination method were 0, 61.90%, 63.80%. Conclusions In the pediatric MP infection, for the high an-tibody titer of MP-IgM antibody, the positive coincidence rate with the colloidal gold method can reach clinical diag-nostic requirements. Clinical physicians according to the age and disease process of patients choose the appropriate method in order to realize the simple, rapid and accurate diagnosis of mycoplasma pneumoniae infection.
