Inhibition of the vasculogenic mimicry formation by targeting fibroblast growth factor receptor in glioma
10.3969/j.issn.1006-5725.2017.11.005
- VernacularTitle:靶向成纤维细胞生长因子受体抑制胶质瘤中血管生成拟态的生成
- Author:
Xiao LI
;
Yun WANG
;
Taoliang CHEN
;
Min HUANG
;
Yiquan KE
- Keywords:
Glioma;
Fibroblast growth factor receptor;
BGJ398;
Vasculogenic mimicry
- From:
The Journal of Practical Medicine
2017;33(11):1735-1738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of fibroblast growth factor receptor(FGFR)inhibitor BGJ398 on the vasculogenic mimicry(VM)formation of glioma cells. Methods The phosphor-FGFR(pFGFR)was de-tected by Western blot,the expressions of MMP2 and MMP14 were detected by Western blot and immunocytochem-istry;the VM formation of U87MG and U251MG was tested by tube formation assay;subcutaneously implanted tu-mor model in nude mouse was established and tumor sections were CD34/PAS double-stained to detect the forma-tion of VM in vivo. Results Western blot showed that pFGFR in the experimental groups decreased significantly (P < 0.05);western blot and immunohistochemical staining showed that the expression of MMP2 and MMP14 in the experimental groups decreased significantly compared to the control group. In the tube formation assay ,the tube formation of U87MG and U251MG cells were restrained. In the subcutaneously implanted tumor model ,the VM number of the experimental group(13.85 ± 3.96)was significantly lower than that in the control group(26.40 ± 5.06,P < 0.05). Conclusions In vivo and in vitro experiments confirmed that BGJ398 can inhibit the activa-tion of FGFR,and inhibit the VM formation of glioma cells. These indicate FGFR signaling pathway is involved in the formation of VM.
