The effect of the recombinant human resistin on lipid metabolism by AMPK pathway in HepG2 cells
10.3969/j.issn.1006-5725.2017.11.007
- VernacularTitle:抵抗素介导AMPK信号通路对HepG2细胞脂质代谢的影响
- Author:
Zhaofan LUO
;
Fangping LI
;
Hua CHENG
- Keywords:
Resistin;
FFAs;
Lipid;
AMPK;
HepG2 Cell
- From:
The Journal of Practical Medicine
2017;33(11):1743-1747
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of rh-resistin on lipid metabolism in HepG2 cells and to elucidate its relation to AMPK pathway. Methods We treated the HepG2 cells with 50 ng/ml rh-resistin and 0.5 mmol/L palmitic acid,used siRNA technique to inhibite α2 subunite expression of AMPK in HepG2 cells and quantitative RT-PCR to detect ACC1,ACC2,and HL mRNA expression levels of related lipid metabolism genes. The P-AMPK-Thr172 of AMPK and P-ACC-Ser79 of ACC were determined by Western blotting. The Lipid accumu-lation in cells was determined by images of Laser Scanning Confocal Microscope after Nile red staining. Results Rh-resistin decreased the AMPKα2,HL mRNA expressions and the phosphorylation level of AMPK and ACC in both basal and insulin-stimulated conditions (P < 0.05),had no influence on ACC2 mRNA expressions (P >0.05),while it increased ACC2 mRNA expressions and cytoplasmic lipid droplets in the same conditions (P <0.05). Conclusion Rh-resistin may affect lipid metabolism via AMPK pathway with increase of fatty acid synthe-sis and inhibition of triglyceride catabolism,which leading to lipid accumulation in HepG2 cells.
